Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response
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چکیده
منابع مشابه
Biological indicators of stress in pacu (Piaractus mesopotamicus) after capture.
The effects of capture (chasing, netting and air exposure) on cortisol, glucose, chloride, sodium, potassium and calcium concentrations, osmolality, hematocrit, hemoglobin concentration, red blood cells count (RBC) and mean corpuscular volume (MCV) were investigated in pacu (Piaractus mesopotamicus). A total of 132 fish (49.7 +/- 11.7 g) were subjected to capture and 3 minutes air exposure and ...
متن کاملHemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with ...
متن کاملDisease resistance of pacu Piaractus mesopotamicus (Holmberg, 1887) fed with β-glucan.
Effects of β-glucan on innate immune responses and survival were studied in pacu experimentally infected with Aeromonas hydrophila. Fish fed diets containing 0, 0.1% and 1% β-glucan were injected with A. hydrophila. β-glucan enhanced fish survival in both treated groups (26.7% and 21.2% of the control, respectively). Leukocyte respiratory burst and alternative complement pathway activities were...
متن کاملThe effect of hypoxia and recuperation on carbohydrate metabolism in pacu (Piaractus mesopotamicus).
A study of the hematological parameters, glycogen, glucose, and lactate, and the activity of malate and lactate dehydrogenases was carried out in blood and tissues of fishes submitted to two, four, and six hours of hypoxia and recuperation. Only after 4 h of hypoxia was there a drop in liver glucose. Alter 16 h, a drop in lactate and a rise in glucose in practically all tissues signaled a recup...
متن کاملLeukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus.
The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week aft...
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ژورنال
عنوان ژورنال: Frontiers in Genetics
سال: 2020
ISSN: 1664-8021
DOI: 10.3389/fgene.2020.00604